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A9169 Sigma

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   rabbit
application(s)   direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
  western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

purified rabbit IgG

Preparation Note

Prepared by two-step glutaraldehyde method.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

Price and Availability


Detect Your Unseen Protein

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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