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A9169 Sigma

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   rabbit
application(s)   direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
  western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C



purified rabbit IgG

Preparation Note

Prepared by two-step glutaraldehyde method2.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal


Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. 1

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

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IgG fraction of antiserum, buffered aqueous solution

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Safety & Documentation

Safety Information

WGK Germany 

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunohistochemistry, Immunoprecipitation, Molecular biology, Peptide synthesis, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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1. DsbA plays a critical and multifaceted role in the production of secreted virulence factors by the phytopathogen Erwinia carotovora subsp. atroseptica. Coulthurst. SJ. J. Biol. Chem. 283, 23739-23753, (2008)


2. Avrameas, S., et al. Scand. J. Immunol. 7th ed., 8, 7, (1978)

Differential Regulation of Human Papillomavirus Type 8 by Interferon Regulatory Factors 3 and 7 Oldak. M. J. Virol. 277, 178-188, (2011)

RAD51 supports spontaneous non-homologous recombination in mammalian cells, but not the corresponding process induced by topoisomerase inhibitors Arnaudeau, C., et al. Nucleic Acids Res. 29, 662-667, (2001)


Cloning and Molecular Characterization of the Basic Peroxidase Isoenzyme from Zinnia elegans, an Enzyme Involved in Lignin Biosynthesis1 Barcel, A.R., et al. Plant Physiol. 139, 1138-1154, (2005)


Drug delivery by polymeric nanoparticles induces autophagy in macrophages. Eidi, H., et al. Int. J. Pharm. 422, 495-503, (2012)


Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus. Chora S, McDonagh B, Sheehan D, et al. Mar. Environ. Res. 66(1), 95-7, (2008)


Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplasm of elongated spermatids. Reproduction 139, 209-16, (2010)


Effects of combined hormone replacement therapy or its effective agents on the IGF-1 pathway in skeletal muscle. Pöllänen, E., et al. Growth Horm. IGF Res. 20, 372-9, (2010)


The Alpha1a-adrenergic Receptor Occupies Membrane Rafts With Its G Protein Effectors But Internalizes Via Clathrin-coated Pits. Morris, D.P., et al. J. Thorac. Cardiovasc. Surg. 283, 2973-85, (2008)


Targeted Deletion Of Rad9 In Mouse Skin Keratinocytes Enhances Genotoxin-induced Tumor Development. Hu, Z., et al. Cancer Res. 68, 5552-61, (2008)


Plasma membrane domains participate in pH banding of Chara internodal cells. Schmölzer, P.M., et al. Plant Cell Physiol. 52, 1274-88, (2011)


Mouse Rad1 deletion enhances susceptibility for skin tumor development. Han, L., et al. Mol. Cancer 9, 67, (2010)


Alfa-class prefoldin protein UXT is a novel interacting partner of Amyotrophic Lateral Sclerosis 2 (Als2) protein. Enunlu, I., et al. Biochem. Biophys. Res. Commun. 413, 471-5, (2011)


Analysis Of The Genotype And Virulence Of Staphylococcus Epidermidis Isolates From Patients With Infective Endocarditis. Monk, A.B., et al. Infect. Immun. 76, 5127-32, (2008)


CRTAM: A molecule involved in epithelial cell adhesion. Garay, E., et al. J. Cell. Biochem. 111, 111-22, (2010)


Accumulation, Activity And Localization Of Cell Cycle Regulatory Proteins And The Chloroplast Division Protein FtsZ In The Alga Scenedesmus Quadricauda Under Inhibition Vítová, M., et al. Plant Physiol. 49, 1805-17, (2008)


Adeno-associated Virus Small Rep Proteins Are Modified With At Least Two Types Of Polyubiquitination. Farris, K.D., et al. J. Virol. Meth. 84, 1206-11, (2010)


The biliprotein C-phycocyanin modulates the early radiation response: a pilot study. Ivanova, K.G., et al. Mutat. Res. 695, 40-5, (2010)


Immunization of Saimiri sciureus monkeys with Plasmodium falciparum merozoite surface protein-3 and glutamate-rich protein suggests that protection is related to antibody levels. Carvalho LJ, Oliveira SG, Theisen M, et al. Scand. J. Immunol. 59(4), 363-72, (2004)


Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to unilateral ovariectomy. García-López Á, , et al. Gen. Comp. Endocrinol. 174, 326-34, (2011)


Replacement of the C-terminal tetrapeptide (314 PAPV 317 to 314 SSSM 317) in interferon regulatory factor-2 alters its N-terminal DNA-binding activity. J. Biosci. 35, 547-56, (2010)


Characterisation of the FAM69 family of cysteine-rich endoplasmic reticulum proteins. Tennant-Eyles, A.J., et al. Biochem. Biophys. Res. Commun. 406, 471-7, (2011)


Katanin knockdown supports a role for microtubule severing in release of basal bodies before mitosis in Chlamydomonas. Rasi MQ Mol. Biol. Cell 20(1), 379-88, (2009)


Correlation between MMP-9 and extracellular cytokine HMGB1 in prediction of human ischemic stroke outcome. Sapojnikova N, Kartvelishvili T, Asatiani N, et al. Biochim. Biophys. Acta 1842(9), 1379-84, (2014)


Differential hypoxic tolerance is mediated by activation of heat shock response and nitric oxide pathway. Jain K, Suryakumar G, Ganju L, et al. Cell Stress Chaperones 19(6), 801-12, (2014)


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