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A9169 Sigma

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates,
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   rabbit
application(s)   direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
  western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

purified rabbit IgG

Preparation Note

Prepared by two-step glutaraldehyde method2.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. 1

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

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