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A9171 Sigma

Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, CE-CH, Chicken Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:35,000
  dot blot: 1:100,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
  western blot (chemiluminescent): suitable
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

IgY (IgG) isolated from pooled normal chicken serum.

IgY (IgG) isolated from pooled normal chicken serum

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Application

Anti-Chicken IgY (IgG) (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot (1:1000).

Anti-chicken IgY (IgG) (whole molecule) −alkaline phosphatase antibody can be used in direct ELISA (diluted 1:35,000) and western blotting (diluted 1:160,000).

ELISA was performed on protein lysates from quail eggs using alkaline-phosphatase conjugated anti-chicken IgY at a dilution of 1:1000 in 0.2% milk.

General description

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications . Anti-Chicken IgY (IgG) (whole molecule)-Alkaline Phosphatase antibody is specific for chicken IgY (IgG).

Immunoglobulin Y (IgY) is also known as egg yolk immunoglobulin and is found mainly in birds, amphibians and reptiles. IgY cannot activate mammalian complement system and cannot bind to mammalian rheumatoid factors, and protein A or G. IgY facilitates an essential role in veterinary science, functional food and bio-products. It also has a crucial role in controlling infectious diseases of bacterial and viral origin. Anti-chicken IgY (IgG) (whole molecule) −alkaline phosphatase antibody can be used as a primary antibody in immunohistology (diluted 1:40). It can also be used in dot blot (diluted 1:50,000-1:100,000). Rabbit anti- chicken IgY−alkaline phosphatase antibody reacts specifically with chicken IgY (IgG).

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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