Annexin V FITC Conjugate from human placenta

Properties

Related Categories	Apoptosis Detection, Apoptosis Reagents, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Other Apoptosis Related Reagents, Reagents More...
Quality Level	PREMIUM
form	buffered aqueous solution
extent of labeling	1-2 mol FITC per mol annexin V
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... ANXA5(308)

Description

Application

For detection of apoptotic cells by flow cytometry or fluorescence microscopy.

Packaging

Package size based on protein content

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, containing 100 mM NaCl.

Biochem/physiol Actions

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg²⁺ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.