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A9378 Sigma

L-Amino Acid Oxidase from Crotalus adamanteus

Type IV, ≥3.0 units/mg protein (biuret), aqueous suspension

Synonym: L-AAO, L-Amino acid:oxygen oxidoreductase (deaminating)

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Properties

Related Categories 1.4.x.x Acting on CH-NH2, 1.x.x.x Oxidoreductases, Amino Acid Metabolism, Biochemicals and Reagents, Cell Biology,
type   Type IV
form   aqueous suspension
contains   toluene as preservative
concentration   ≥5.0 mg/mL
solubility   H2O: soluble1.0 mg/mL, clear
storage temp.   2-8°C

Description

Packaging

2, 5, 10 mg in glass bottle

Package size based on protein content

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Unit Definition

One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 °C. (L-Leucine is deaminated at the same rate at pH 7.8 at 37 °C.)

Application

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. 35S dimethylsulfoniopropionate (DMSP) has been synthesized chemically from 35S L-methionine using LAAO from Sigma to form 35S 3-methiolpropionate.

Biochem/physiol Actions

L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGI
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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