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A9414 Sigma

Agarose, low gelling temperature

BioReagent, for molecular biology

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,
grade   for molecular biology
product line   BioReagent
foreign activity   DNase, RNase, NICKase, none detected

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Modified to produce lower gelling and melting points and gives convenient recovery and purification of DNA from low melting agarose gels. Radiolabeled DNA probes can be prepared directly in remelted agarose using DNA gel slices cut from low-melting agarose gels.
Cloning of restriction fragments into plasmids can be conveniently carried out directly in remelted agarose using DNA gel slices cut from low-melting agarose gels.
Gels exhibit low background fluorescence using ethidium bromide staining.

Price and Availability

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Safety & Documentation

Protocols & Articles

Related Content

Agarose Selection Guide

From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers

References

Set your institution to view full text papers.

A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Wieslander, L. Anal. Biochem. 98, 305, (1979)

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Struhl, K. Biotechniques 3, 452, (1985)

Consumption of a dark roast coffee decreases the level of spontaneous DNA strand breaks: a randomized controlled trial. Bakuradze T, Lang R, Hofmann T, et al. Eur. J. Nutr. 54(1), 149-56, (2015)

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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity (addendum). Feinberg, A.P. and Vogelstein, B. Anal. Biochem. 137, 266-267, (1984)

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Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching. Jérôme Bove et al Plant Physiol. 147, 1646-58, (2008)

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Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. Advani A, Connelly KA, Yuen DA, et al. PLoS ONE 6(10), e24695, (2011)

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Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres. Skala MC, Crow MJ, Wax A, et al. Nano Lett. 8(10), 3461-7, (2008)

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Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Ruparelia AA, Oorschot V, Vaz R, et al. Acta Neuropathol. 128(6), 821-33, (2014)

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Doxycycline-mediated inhibition of choroidal neovascularization. Samtani S Invest. Ophthalmol. Vis. Sci. 50(11), 5098-106, (2009)

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Regression-based identification of behavior-encoding neurons during large-scale optical imaging of neural activity at cellular resolution. Miri A, Daie K, Burdine RD, et al. J. Neurophysiol. 105(2), 964-80, (2011)

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ATP-dependent but proton gradient-independent polyphosphate-synthesizing activity in extraradical hyphae of an arbuscular mycorrhizal fungus. Tani C, Ohtomo R, Osaki M, et al. Appl. Environ. Microbiol. 75(22), 7044-50, (2009)

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Effect of cold storage on collagen-based hydrogels for the three-dimensional culture of adipose-derived stem cells. Sancho A, Vázquez L, and De-Juan-Pardo EM Biofabrication 6(3), 035017, (2014)

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Unique function of Kinesin Kif5A in localization of mitochondria in axons. Campbell PD, Shen K, Sapio MR, et al. J. Neurosci. 34(44), 14717-32, (2014)

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Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform. Sivasubramaiyan K, Totey S, Bhat V, et al. J. Biomed. Sci. 16, 88, (2009)

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Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY , (1989), 6

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