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A9414 Sigma

Agarose, low gelling temperature

BioReagent, for molecular biology

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,
grade   for molecular biology
product line   BioReagent
EEO   ≤0.10
mp   ≤65 °C ( at a 1.5% gel)
transition temp   congealing temperature 26-30 °C
gel strength   ≥200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.10%
foreign activity   DNase, RNase, NICKase, none detected

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Modified to produce lower gelling and melting points and gives convenient recovery and purification of DNA from low melting agarose gels. Radiolabeled DNA probes can be prepared directly in remelted agarose using DNA gel slices cut from low-melting agarose gels.
Cloning of restriction fragments into plasmids can be conveniently carried out directly in remelted agarose using DNA gel slices cut from low-melting agarose gels.
Gels exhibit low background fluorescence using ethidium bromide staining.

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

Price and Availability


Hybridization
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

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