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A9452 Sigma

Monoclonal Anti-Goat/Sheep IgG–Peroxidase antibody produced in mouse

clone GT-34, purified immunoglobulin, lyophilized powder

Synonym: Monoclonal Anti-Goat/Sheep IgG

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, GM-GO, Goat Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   GT-34, monoclonal
form   lyophilized powder
species reactivity   goat, bovine, sheep
does not react with   feline, mouse, rabbit, pig, guinea pig, human, rat, chicken, canine, horse
packaging   vial of 0.5 mL
application(s)   direct ELISA: 1:30,000
  dot blot: 1:160,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
isotype   IgG1
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Application

ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.

Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Peer-Reviewed Papers
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