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A9460 Sigma

Annexin V from human placenta

≥90% (SDS-PAGE), buffered aqueous solution

Synonym: Annexin V, Calphosbindin I, Lipocortin V, PAP-1

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Properties

Related Categories Apoptosis Detection, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Reagents More...
assay   ≥90% (SDS-PAGE)
form   buffered aqueous solution
mol wt   mol wt 33 kDa (four subunits)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ANXA5(308)

Description

Packaging

0.1 mg in poly bottle

Package size based on protein content

Physical form

Solution in 40 mM Tris-HCl, pH 7.5, containing 150 mM NaCl, 1 mM DTT and 0.05% sodium azide.

Biochem/physiol Actions

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg2+ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

Price and Availability


Flow Cytometry Instruments
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
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