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A9521 Sigma

Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody produced in rabbit

IgG fraction of antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Miscellaneous, Primary Antibodies More...
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   lyophilized powder
species reactivity   yeast
application(s)   immunoelectrophoresis: suitable
  indirect ELISA: 1:15,000-1:30,000
conjugate   unconjugated
shipped in   ambient
storage temp.   2-8°C

Description

Immunogen

G-6-PDH from Baker′s yeast S. cerevisiae.

Reconstitution

Reconstitute with 2mL deionized water.

Physical form

Lyophilized from 0.01 M phosphate buffered saline.

Application

Rabbit Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used for western blot assays. The antibody can also be used for indirect ELISA (1:15,000-1:30,000) and immunoelectrophoresis.

Yeast cell extracts were analyzed by western blot and equal loading was determined by probing with rabbit anti-Glucose-6-Phosphate Dehydrogenase antibody.

General description

G-6-PDH catalyzes the NADP+(or NAD+)-dependent oxidation of D-glucose-6-phosphate to 6-phospho-D-glucono-1,5-lactone. This reaction is the rate limiting step of the pentose phosphate pathway.
Yeast G-6-PDH is often used as a loading control in several immunoassays.

The product is specific for G-6-PDH as determined by immunoelectrophoresis. No reaction is observed with other proteins in a crude Baker′s yeast extract.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

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