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A9528 Sigma

Amphotericin B solubilized

powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym: Amphotericin B from Streptomyces sp., Fungizone

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Properties

Related Categories Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal,
Quality Level   PREMIUM
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
sterility   γ-irradiated
product line   BioXtra
form   powder
solubility   sterile water: soluble20 mg/mL (as a stock solution. Stock solutions should be stored at −20 °C. Stable at 37 °C for 3 days.)
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Packaging

1, 5 g in glass bottle

50, 100, 500 mg in glass bottle

Solubilized Amphotericin B is packaged in a bottomless glass bottle, with the contents inside an inserted fused cone.

Preparation Note

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted at 25mg/10mL of sterile water, there is no need to filter sterilize.

Protocols & Applications

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Standard component

Description

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Amphotericin B ~ 45 % SDS    
Sodium deoxycholate ~ 35 % SDS    
Sodium phosphate balance SDS    
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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