|Related Categories||Agarose, Agarose Gel, Core Bioreagents, Life Science Reagents for Cloning, Life Science Reagents for DNA/RNA Electrophoresis,|
|impurities||≤10% moisture content|
|transition temp||gel point 36 °C (± 1.5 °C)|
|foreign activity||DNase, RNase, none detected|
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.
Certificate of Analysis
Certificate of Origin
For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Sigma's GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and pu...
Keywords: Amplification, Anticoagulants, Catalog, Cell disruption, Centrifugation, Degradations, Digestions, Evaporation, Help, Instructions, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Type
For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information.
Sigma’s GenElute Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whol...
Sigma’s GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silic...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Catalog, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Help, Instructions, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing
From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation
Residues in the 11 A channel of histone deacetylase 1 promote catalytic activity: implications for designing isoform-selective histone deacetylase inhibitors. Weerasinghe SV J. Med. Chem. 51(18), 5542-51, (2008)
Mousepox detected in a research facility: case report and failure of mouse antibody production testing to identify Ectromelia virus in contaminated mouse serum. Labelle P Comp. Med. 59(2), 180-6, (2009)
Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement. Müller TK, Cao P, Ewert S, et al. J. Chromatogr. A 1285, 97-109, (2013)
Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine. Fernández E, Toledo JR, Méndez L, et al. PLoS ONE 8(2), e56417, (2013)
Magnetic resonance imaging (MRI) markers for MRI-guided high-dose-rate brachytherapy: novel marker-flange for cervical cancer and marker catheters for prostate cancer. Schindel J, Muruganandham M, Pigge FC, et al. Int. J. Radiat. Oncol. Biol. Phys. 86(2), 387-93, (2013)
Hydrolysis of chickpea proteins with Flavourzyme immobilized on glyoxyl-agarose gels improves functional properties. del Mar Yust M, del Carmen Millán-Linares M, Alcaide-Hidalgo JM, et al. Food Sci. Technol. Int. 19(3), 217-23, (2013)
A non-exothermic cell-embedding tissue-mimicking material for studies of ultrasound-induced hyperthermia and drug release. Mylonopoulou E, Bazán-Peregrino M, Arvanitis CD, et al. Int. J. Hyperthermia 29(2), 133-44, (2013)
Purification of human immunoglobulin G autoantibodies to tumor necrosis factor using affinity chromatography and magnetic separation. Sennikov SV, Golikova EA, Kireev FD, et al. J. Immunol. Methods 390(1-2), 92-8, (2013)
Removable silicon insertion stiffeners for neural probes using polyethylene glycol as a biodissolvable adhesive. Felix S, Shah K, George D, et al. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2012, 871-4, (2012)
The use of agarose microwells for scalable embryoid body formation and cardiac differentiation of human and murine pluripotent stem cells. Dahlmann J, Kensah G, Kempf H, et al. Biomaterials 34(10), 2463-71, (2013)
A biomimetic Protein G affinity adsorbent: an Ugi ligand for immunoglobulins and Fab fragments based on the third IgG-binding domain of Protein G. El Khoury G and Lowe CR J. Mol. Recognit. 26(4), 190-200, (2013)
Cat and dog primordial follicles enclosed in ovarian cortex sustain viability after in vitro culture on agarose gel in a protein-free medium. Fujihara M, Comizzoli P, Wildt DE, et al. Reprod. Domest. Anim. 47 Suppl 6, 102-8, (2012)
Combination of fibrin-agarose hydrogels and adipose-derived mesenchymal stem cells for peripheral nerve regeneration. Carriel V, Garrido-Gómez J, Hernández-Cortés P, et al. J. Neural Eng. 10(2), 026022, (2013)
WFDC1/ps20 is a novel innate immunomodulatory signature protein of human immunodeficiency virus (HIV)-permissive CD4+ CD45RO+ memory T cells that promotes infection by upregulating CD54 integrin expression and is elevated in HIV type 1 infection. R. Alvarez et al J. Virol. 82, 471-86, (2008)
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|DNTP100||Deoxynucleotide Set, 100 mM, Individual dNTPs for routine PCR; 0.25 mL each|
|A2549||Tape, autoclave indicator, Sigmaware™, impregnated to show diagonal lines|
|E7637||Ethidium bromide, BioReagent, for molecular biology, powder|
|T1503||Trizma® base, Primary Standard and Buffer|
|T6066||Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)|
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