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A9542 Sigma

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Peroxidase Labeled Antibodies, Primary Antibodies, RAE-RAX,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   rat
application(s)   direct ELISA: 1:40,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500
  western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

purified rat IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

ELISA was performed to detect specific antibodies in rat serum using HRP-conjugated rabbit anti-rat IgG as the secondary antibody at a dilution of 1:10000.

Rabbit Anti-Rat IgG (whole molecule)-Peroxidase antibody can be used for western blotting assays at 1:8,000. The antibody can also be used for direct ELISA (1:40,000) and IHC (1:500) applications.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Rabbit Anti-Rat IgG (whole molecule)-Peroxidase antibody binds to rat IgG.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

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