Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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MDL number MFCD00162437
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Properties
| Related Categories | Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, |
| antibody form | affinity isolated antibody |
| clone | polyclonal |
| form | buffered aqueous solution |
| application(s) | direct ELISA: 1:55,000 |
| dot blot: 1:40,000 | |
| dot blot: 1:40,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 |
Description
Other Notes
No cross-reaction with mouse and rat IgG.
Immunogen
Human IgG, Fc fragment
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% BSA, 1 mM MgCl2 and 15 mM sodium azide
Application
A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) assay was performed on human platelet eluates using alkaline phosphatase-conjugated goat anti-human, Fc specific, antibody as the secondary with a 30 minute incubation. 1
Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions . The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections).
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders 2,3. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG.
Price and Availability
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