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A9594 Sigma

Monoclonal ANTI-FLAG® M2-Cy3 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate,)

Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG Antibody Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   M2, monoclonal
form   buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate,)
concentration   ~1 mg/mL
application(s)   direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype   IgG1
conjugate   CY3 conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Application

For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Packaging

0.2, 1 mg in poly bottle

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Cy3 is a trademark of GE Healthcare

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tools for Large Molecule BioAnalysis

Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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