FLAG® M2-Cy3™ monoclonal antibody (DYKDDDDK tag) produced in mouse

Properties

Related Categories	Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, Clone Index, FK-FO, FLAG Antibody Conjugates, FLAG System, M, Molecular Biology, Primary Antibodies, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis More...
antibody form	purified immunoglobulin
clone	M2, monoclonal
form	buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate,)
concentration	~1 mg/mL
application(s)	direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype	IgG1
shipped in	dry ice
storage temp.	−20°C

Show More (9)

Description

Application

For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.