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A9647 Sigma

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

• Bovine Serum Albumin has been used as a supplement in the medium prepared for culturing of oligodendrocytes.
• It has been used for the dilution of primary antibody solution in immunohistochemistry procedure.
• It has been used as a standard in the Bradford method of protein quantification.
• It has been used in the blocking and permeabilization buffer during immunofluorescence studies.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the molecular weight of Product A9647, Albumin from bovine serum?
The molecular weight of BSA is 66,430.
How can the concentration of a solution of Product A9647, Albumin from bovine serum, be quantified?
The concentration of a solution of BSA can be measured by using the extinction coefficient (E1%) of 6.67. The 1% refers to a solution that is 1% weight-to-volume (i.e., 10 mg/mL).
What is the pH of a solution of Product A9647, Albumin from bovine serum?
The pH of a 1% solution is approximately 7.0, according to the product specifications.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
I need help selecting an Albumin from bovine serum (BSA) product, is there a guide to help me choose the best product?
Yes,  a selection guide to our BSA products can be found at the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/albumin_application_selection_guide1.Par.0001.File.tmp/albumin_application_selection_guide1.pdf
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Protocols & Articles

Protocols

4-Chloro-1-Naphthol Solution Protocol

Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotti...
Keywords: Western blot

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

This procedure may be used for Alcohol Dehydrogenase products, except for insoluble forms of Alcohol Dehydrogenase (Catalog No. A2529).
Keywords: Extinction coefficient

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Peer-Reviewed Papers
15

References

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