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A9669 Sigma

Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, HU-HZ, Human IgA Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:35,000
  dot blot: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Purified human IgA

Specificity

The conjugate is specific for human IgG. No cross-reaction with Mouse and Rat IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Application

Anti-Human IgA (α-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product may also be used for dot blot (1:30,000) and immunohistochemistry (at 1:100 dilutions, using formalin-fixed, paraffin-embedded sections).

Human milk samples were analyzed for the presence of IgA antibodies specific to certain antigens of H. pylori by western blot using alkaline-phosphate-conjugated goat anti-human IgA antibody.

Milk samples from 10 mothers were probed for bound milk IgA by western blot using alkaline-phosphatase goat anti-human IgA antibody.Elisa Assays were performed using alkaline phosphatase conjugated gaot anti-human IgA chain specific antibody (1:1000) to detect IgA immunoglobulins in serum and seminal plasma samples from infertile men.

General description

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines
Anti-Human IgA (α-chain specific)-Alkaline Phosphatase antibody is specific for human IgA when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins. The product does not react with mouse and rat IgGs.

Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgA is determined by Enzyme Linked Immunosorbent Assay (ELISA). Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

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RIDADR 
NONH for all modes of transport
WGK Germany 
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Protocols & Articles

Articles

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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