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A9688 Sigma

Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
species reactivity   mouse
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody has been used for ELISA and immunofluorescence assays. The antibody can also be used for IHC (1:50) and western blot (1:30,000) applications.

Mouse plasma antibody isotype determination was performed by ELISA using alkaline phopshatase-conjugated goat anti-mouse IgM as the secondary antibody.

General description

Binds mouse IgM; does not bind other mouse Igs.

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for mouse IgM when tested against purified mouse IgA, IgG and IgM myeloma proteins.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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