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A9776 Sigma

α-Actinin from chicken gizzard

~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension

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Properties

Related Categories Actin/Actin-binding Proteins, Actinin, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
assay   ~80% α-actinin basis (SDS-PAGE)
form   ammonium sulfate suspension
mol wt   mol wt 100 kDa
storage temp.   2-8°C
Gene Information   chicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918)
human ... ACTN1(87)
mouse ... ACTN1(109711)
rat ... ACTN1(81634)

Description

Biochem/physiol Actions

Cross-linking protein in the actin cytoskeleton

Packaging

5 mg in poly bottle

Package size based on protein content

Preparation Note

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.

Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

Physical form

Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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