Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories	Alphabetical Index, Antibodies, CE-CH, Chicken Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies More...
antibody form	IgG fraction of antiserum
clone	polyclonal
form	buffered aqueous solution
application(s)	direct ELISA: 1:30,000
	dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate.
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.
shipped in	dry ice
storage temp.	−20°C

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Description

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution. ¹