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A9891 Sigma

7-Amino-4-methylcoumarin

Chromophore for substrates

Synonym: Coumarin 120

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Properties

Related Categories Activity, Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,
description   chromophore for enzyme substrates
InChI Key   GLNDAGDHSLMOKX-UHFFFAOYSA-N
mp   223-226 °C(lit.)
fluorescence   λex 365 nm; λem 440 nm in ethanol(lit.)
storage temp.   2-8°C

Description

Application

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Packaging

1 g in poly bottle

250, 500 mg in poly bottle

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Protocols & Applications

Methods of glycosylated protein analysis by Mass Spectrometry

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Protocols

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Peer-Reviewed Papers
15

References

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