Synonym: Coumarin 120
|Related Categories||Activity, Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,|
|description||chromophore for enzyme substrates|
|fluorescence||λex 365 nm; λem 440 nm in ethanol(lit.)|
Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.
Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.
1 g in poly bottle
250, 500 mg in poly bottle
Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).
Certificate of Analysis
Certificate of Origin
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26-36|
In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
BioFiles 2011, 6.3, 23.
Keywords: Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Microscopy, Neurotransmission, Sol-gel
1. OBJECTIVE To standardize a procedure for determining the enzymatic activity of Cathepsin B.
Keywords: Enzyme activity, Enzymology
Khammungkhune, S. and Sigler, G. Synthesis, 614, (1980)
Online nanoliquid chromatography-mass spectrometry and nanofluorescence detection for high-resolution quantitative N-glycan analysis. Kalay H, Ambrosini M, van Berkel PH, et al. Anal. Biochem. 423(1), 153-62, (2012)
Characterization of 7-amino-4-methylcoumarin as an effective antitubercular agent: structure-activity relationships. Tandon R, Ponnan P, Aggarwal N, et al. J. Antimicrob. Chemother. 66(11), 2543-55, (2011)
A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin. Choi MG, Lee E, Chung HS, et al. BMB Rep. 44(7), 458-61, (2011)
Endostatin expression in neurons during the early stage of cerebral ischemia is associated with neuronal apoptotic cell death in adult hypertensive rat model of stroke. Hou Q, Ling L, Wang F, et al. Brain Res. 1311, 182-8, (2010)
Optimized conditions for high-performance liquid chromatography analysis of oligosaccharides using 7-amino-4-methylcoumarin as a reductive amination reagent. M. Yodoshi, et al., J. Chromatogr. A. 1203, 137-145, (2008)
Solvent effect on the absorption spectra of coumarin 120 in water: A combined quantum mechanical and molecular mechanical study. Sakata T, Kawashima Y, and Nakano H J. Chem. Phys. 134(1), 014501, (2011)
Kallikrein-related peptidase-8 (KLK8) is an active serine protease in human epidermis and sweat and is involved in a skin barrier proteolytic cascade. Eissa A, Amodeo V, Smith CR, et al. J. Biol. Chem. 286(1), 687-706, (2011)
High-performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect fluorescence detection. Al-Kindy SM and Miller JN Biomed. Chromatogr. 23(2), 166-9, (2009)
Antimicrobial activity of an endophytic Xylaria sp.YX-28 and identification of its antimicrobial compound 7-amino-4-methylcoumarin. Liu X, Dong M, Chen X, et al. Appl. Microbiol. Biotechnol. 78(2), 241-7, (2008)
Aldrich MSDS 1, 92:B / Corp MSDS 1 (1), 190:D / FT-IR 1 (2), 328:D / FT-IR 2 (2), 2914:B / FT-NMR 1 (2), 1318:C / NMR-Reference 2 (2), 313:B / RegBook 1 (2), 1945:O / Sigma FT-IR 1 (2), 98:C / Structure Index 1, 313:A:5
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