A9891 Sigma

7-Amino-4-methylcoumarin

Chromophore for substrates

DOWNLOAD MSDS (PDF)

Synonym: Coumarin 120

Properties

Related Categories Activity, Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,
description   chromophore for enzyme substrates
mp   223-226 °C(lit.)
fluorescence   λex 365 nm; λem 440 nm in ethanol(lit.)
storage temp.   2-8°C

Description

Application

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Packaging

1, 5 g in poly bottle

250, 500 mg in poly bottle

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Protocols & Applications

Methods of glycosylated protein analysis by Mass Spectrometry

Price and Availability

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