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  • A9935 - Anti-Human IgM (μ-chain specific)−Agarose antibody produced in goat

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A9935 Sigma

Anti-Human IgM (μ-chain specific)−Agarose antibody produced in goat

affinity isolated antibody, PBS suspension

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Properties

Related Categories Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization, Antibody-Agaroses,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   PBS suspension
application(s)   Ouchterlony double diffusion: suitable
  immunoelectrophoresis: suitable
capacity   2-3 mg/mL, resin binding capacity (human IgM)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Other Notes

Antibody adsorbed with mouse and rat IgG

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Goat polyclonal anti-Human IgM (μ-chain specific)−Agarose antibody conjugate is used as an immuno-adsorbent to affinity purify antibodies, to remove species specific cross-reacting antibodies or to remove contaminating antibodies from an antiserum preparation. It has been used for IgM depletion from normal human serum from fasted patients.

General description

Goat polyclonal anti-Human IgM (μ-chain specific)−Agarose antibody specific for human IgM when tested against purified human IgA, IgG, IgM, and kappa and lambda light chains. No reactivity with mouse or rat IgG is observed by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Cyanogen bromide activated cross-linked beaded agarose is frequently conjugated to proteins such as immunoglobulins to facilitate their use in physicochemical processes such as precipitation, absorption and clarification.

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