Synonym: AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,|
Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide
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Liquid Substrate System
for peroxidase detection
sufficient for 200 tests
Certificate of Analysis
Certificate of Origin
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26-36|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions:
Keywords: Enzyme activity, Extinction coefficient
Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient
Horseradish peroxidase: a study of the kinetics and the determination of optimal reaction conditions, using hydrogen peroxide and 2,2'-azinobis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) as substrates (author's transl). Gallati, V.H. J. Clin. Chem. Clin. Biochem. 17, 1, (1979)
Differences in the phenolic composition and antioxidant properties between Vitis coignetiae and Vitis vinifera seeds extracts. Weidner S, Rybarczyk A, Karamać M, et al. Molecules 18(3), 3410-26, (2013)
Complex formation, thermal behavior and stability competition between Cu(II) ion and Cu(0) nanoparticles with some new azo dyes. Antioxidant and in vitro cytotoxic activity. Gaber M, El-Sayed YS, El-Baradie KY, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 107, 359-70, (2013)
Dorsamin-A's, glycerolipids carrying a dehydrophenylalanine ester moiety from the seed-eating larvae of the bruchid beetle Bruchidius dorsalis. Hirose Y, Ohta E, Kawai Y, et al. J. Nat. Prod. 76(4), 554-8, (2013)
An 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)-immobilized electrode for the simultaneous detection of dopamine and uric acid in the presence of ascorbic acid. Chih YK and Yang MC Bioelectrochemistry 91, 44-51, (2013)
Characterization of interdigitated electrode structures for water contaminant detection using a hybrid voltage divider and a vector network analyzer. Rodríguez-Delgado JM, Rodríguez-Delgado MM, Mendoza-Buenrostro C, et al. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2012, 558-61, (2012)
Supercritical carbon dioxide extraction of seed oil from winter melon (Benincasa hispida) and its antioxidant activity and fatty acid composition. Bimakr M, Rahman RA, Taip FS, et al. Molecules 18(1), 997-1014, (2013)
Characterization and biological studies on Co(II), Ni(II) and Cu(II) complexes of carbohydrazones ending by pyridyl ring. Abu El-Reash GM, El-Gammal OA, Ghazy SE, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 26-34, (2013)
Influence of drying and cooking process on the phytochemical content, antioxidant and hypoglycaemic properties of two bell Capsicum annum L. cultivars. Loizzo MR, Pugliese A, Bonesi M, et al. Food Chem. Toxicol. 53, 392-401, (2013)
Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extracts of microalgae and cyanobacteria. Guedes AC, Amaro HM, Gião MS, et al. Food Chem. 138(1), 638-43, (2013)
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