Alkaline Phosphatase Blue Microwell Substrate sufficient for 400 mL working substrate

Properties

Related Categories	Alkaline Phosphatase, Alphabetical Listing, BCIP/NBT Substrate Systems, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates, ELISA Substrates, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Liquid Substrate Systems More...
usage	sufficient for 400 mL working substrate
storage temp.	2-8°C

Description

Application

Prior to reaction with alkaline phosphatase, the BCIP reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Price and Availability

Documents

Certificate of Analysis

Certificate of Origin

AB0100 - Datasheet (74 KB)

Safety Information

Safety Information for this product is unavailable at this time.

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

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Alkaline Phosphatase Blue Microwell Substrate

sufficient for 400 mL working substrate