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AMPD1 Sigma

DNase I

Amplification Grade

Synonym: Deoxyribonuclease I

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Description

Frequently Asked Questions

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Application

Deoxyribonuclease I (DNase I) is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.

Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers.

Features and Benefits

• Suitable for the elimination of DNA from RNA
• Minimal RNase activity available
• Optimized 10× reaction buffer and Stop Solution for complete inactivation of DNase I

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Unit Definition

One unit completely digests 1 μg of plasmid DNA to oligonucleotides in 10 min. at 37 °C.

General description

Deoxyribonuclease I (DNase I) is an endonuclease isolated from bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity, and is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction).

DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.

No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers.

Price and Availability

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PCR Technical Manual

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Protocols & Articles

Articles

Transplex® RNA Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues.

The TransPlex Complete Whole Transcriptome Amplification (WTA2 ) Kit effectively amplifies intact and highly degraded RNA. To benchmark maintenance of representative RNA levels during amplification, ...
Ken Heuermann and Brian Ward
LSI Edition 25
Keywords: Amplification, Applications, Cancer, Chromatin immunoprecipitation, Clinical, Company, DNA microarrays, Degradations, Diagnostic, Drug discovery, Gene expression, Genetic, Genomics, Methods, Microarray Analysis, Molecular biology, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Proteomics, Reductions, Sequencing, Spectroscopy, Transcription

Protocols

Reverse Transcription Using ReadyScript cDNA Synthesis RDRT Protocol

Preparation Place components on ice. Mix, and then briefly centrifuge to collect contents at the bottom of the tube

SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantit...

TransPlex® Whole Transcriptome Amplification Protocol

TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3'-bias. Microgram quantities of ...
Keywords: Amplification, Centrifugation, Cloning, Condensations, DNA purification, Gene expression, Genomics, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Sequencing

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Peer-Reviewed Papers

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