• USA Home
  • APOAC - Annexin V-Cy3 Apoptosis Detection Kit

EMAIL THIS PAGE TO A FRIEND
APOAC Sigma

Annexin V-Cy3 Apoptosis Detection Kit

Purchase

Description

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of GE Healthcare

Price and Availability

Kit component only

Description

Product #

Add to Cart

10× Binding buffer 20 mL SDS    

Standard component

Description

Product #

Add to Cart

6-Carboxyfluorescein diacetate 10 mg SDS C5041
Annexin V-Cy3.18 Conjugate from human placenta 10 μg SDS A4963
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?