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APOAC Sigma

Annexin V-Cy3 Apoptosis Detection Kit

Properties

Related Categories Apoptosis Detection, Apoptosis and Cell Cycle, Cell Analysis, Cell Biology, Cell Signaling and Neuroscience,
usage   sufficient for 200 tests
shipped in   wet ice
storage temp.   2-8°C

Description

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of GE Healthcare

Price and Availability

Kit component only

Description

Product #

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10× Binding buffer 20 mL MSDS    

Standard component

Description

Product #

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6-Carboxyfluorescein diacetate 10 mg MSDS C5041
Annexin V-Cy3.18 Conjugate from human placenta 10 μg MSDS A4963

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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