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APOAF Sigma

Annexin V-FITC Apoptosis Detection Kit

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

General description

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

Other Notes

Allow all components to reach room temperature before use.

Application

Annexin V-FITC Apoptosis Detection Kit was used:
• in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
• for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
• for indirect measurement of flippase activity.

Price and Availability


Flow Cytometry Instruments

All labs need water

Kit component only

Description

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10× Binding buffer SDS    
Propidium Iodide solution SDS    

Standard component

Description

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AnnexinV-FITC solution SDS A9210
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Apoptosis Detection Kits

Programmed cell death is a fundamental process important in development, as well as a principle mechanism of tumor suppression. Apoptosis is triggered in non-malignant cells as a protective mechanism...
Dalit Weinstein-Fischer, Ph.D., Supervisor of Research & Development; Rina Altman, M.Sc., Scientist of Research & Development; Dorit Zharhary, Ph.D., Director of Research & Development
Biowire Spring 2012, 22–24
Keywords: Antibiotics, Apoptosis, Cancer, Condensations, Flow cytometry, Growth factors, Ligands, Methods, Phosphorylations, Separation, Transcription

Peer-Reviewed Papers
15

References

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