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APOBRDU Sigma

Flow Cytometry Kit for Apoptosis

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Description

Application

The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody. Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA.

Features and Benefits

Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.

Packaging

The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20 °C and APO-PART2 at 2-4 °C

Principle

Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3′-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3′-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.

Price and Availability


Your Key to Success

All labs need water

Standard component

Description

Product #

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Br-dUTP 480 μL SDS    
Fluorescein PRB-1 antibody 300 μL SDS    
Negative control cells 5 mL SDS    
PI/RNase staining buffer 30 mL SDS    
Positive control cells 5 mL SDS    
Reaction buffer .6 mL SDS    
Rinsing buffer 120 mL SDS    
Terminal deoxynucleotidyl transferase (TdT) 45 μL SDS    
Wash buffer 120 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C

Documents

Certificate of Analysis

Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Peer-Reviewed Papers
15

References

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