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B0174 Sigma

AccuTaq LA 10× Buffer

10X Buffer for long and accurate PCR

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Properties

grade   for molecular biology
form   liquid
packaging   vial of 0.5 mL
storage temp.   −20°C

Description

General description

AccuTaq LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq LA DNA polymerase.

Application

AccuTaq LA 10× Buffer was used in PCR reaction mixture for the amplification of human BRCA1 gene.

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Long and Accurate PCR Amplification of DNA D8045 Protocol

Effective denaturation is accomplished by the use of higher temperatures for shorter periods of time or by the use of co-solvents, such as dimethyl sulfoxide. Addition of DMSO in the reaction at a fi...

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...

Peer-Reviewed Papers
15

References

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Description

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D4812 REDAccuTaq® LA DNA Polymerase, High fidelity Taq with inert dye, 10X buffer included

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