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B0271 Sigma

Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse

clone GAL-13, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-β-Galactosidase

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Properties

Related Categories β-Galactosidase Detection, Alphabetical Index, Antibodies, Antibodies to beta-Galactosidase, G-GC,
species reactivity   bacteria
application(s)   dot blot: 1:2,000
clone   GAL-13, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   biotin conjugate

Description

Immunogen

β-galactosidase from E. coli

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Preparation Note

Prepared by conjugation with ε-aminocaproyl biotin.

Application

Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.

Biochem/physiol Actions

β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.

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