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B0287 Sigma

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-FITC

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Fluorescent Labels, Biochemicals and Reagents, FB-FJ,
biological source   mouse
antibody form   purified immunoglobulin
clone   FL-D6, monoclonal
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

FITC-BSA conjugate

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Application

Monoclonal Anti-FITC-Biotin antibody produced in mouse may be used for amplification of immunofluorescence assays and for visualization of immunofluorescent staining by light microscopy at a recommended dilution of 1:400. It was added to Ag-activated beads in flow-based microimmunoassay of parathyroid hormone and IL-5.

General description

Monoclonal Anti-FITC-Biotin antibody produced in mouse reacts with free and conjugated FITC and is highly sensitive method of antigen detection.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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