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B0300 Sigma

Betaine solution

5 M, PCR Reagent

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Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents, PCR Enhancers, PCR Reaction Components,
grade   PCR Reagent
form   liquid
packaging   vial of 1.5 mL
concentration   5 M
color   colorless
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
storage temp.   2-8°C

Description

Application

The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.

Packaging

1.5mL per vial

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Long and Accurate PCR Amplification of DNA D8045 Protocol

Effective denaturation is accomplished by the use of higher temperatures for shorter periods of time or by the use of co-solvents, such as dimethyl sulfoxide. Addition of DMSO in the reaction at a fi...

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:

Quantitative Probe Based PCR for Gene Expression

In recent years Quantitative PCR has reached a level of sensitivity, accuracy, and ease to support use as a routine assay for measuring gene level expression. The field of cancer research is currentl...

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Peer-Reviewed Papers
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