B0300 Sigma

Betaine solution

5 M, PCR Reagent

Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents, PCR Enhancers, PCR Reaction Components,
grade   PCR Reagent
packaging   vial of 1.5 mL
concentration   5 M
foreign activity   DNase, RNase, none detected
storage temp.   2-8°C

Description

Application

The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.1

Packaging

1.5mL per vial

Price and Availability

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