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B0555 Sigma

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)−Biotin Conjugate antibody produced in mouse

clone 260.4, KDR-2, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-KDR, Anti-VEGFR-2, Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse

Description

Immunogen

purified recombinant human extracellular VEGF receptor-2 (KDR).

General description

Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. VEGF R2 has the ability to form heterodimers with VEGF R1 and VEGF R3. The binding of VEGF to VEGF R2 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis. Due to its role in promoting migration and angiogenesis, VEGF R2 has been implicated in breast and prostate cancer. Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody is purified from ascites fluid, and conjugated withSulfo-NHS-LC-Biotin. It is specific for human VEGF receptor2. It does not cross react with Flt1, Flt4 or platelet-derived growth factor receptor β (PDGF-R1).

Antigen Background

The KDR receptor (VEGFRa), defining marker of hematopoietic stem cells (HSCs), distinguishes pluripotent HSCs from lineage-committed progenitor HSCs.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

Reacts specifically with human VEGF receptor-2. This clone has also been termed 260.4. It does not recognize Flt1 (VEGFR-1), Flt4 (VEGFR-3), or platelet-derived growth factor receptor (PDGF-R).

Application

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody may be used for immunoblotting at a minimum working dilution of 1:1000. The antibody was used for flow cytometry using endothelial progenitor cells from mice.

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