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B0556 Sigma

Monoclonal Anti-B23 antibody produced in mouse

clone FC82291, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-NPM, Anti-Nucleophosmin

Properties

Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle,
species reactivity   hamster, bovine, monkey, kangaroo rat, mouse, human, canine, rat
application(s)   immunocytochemistry: suitable using 2% formaldehyde-acetone or 10% formalin/methanol-1% NP-40
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 0.2-0.4 μg/mL using a whole extract of 3T3 (mouse fibroblasts) cells
clone   FC82291, monoclonal
concentration   ~0.5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 37 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NPM1(4869)
mouse ... Npm1(18148)
rat ... Npm1(25498)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat B23 (nucleophosmin, NPM, numatrin).

General description

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase A activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E.
Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

It detects both the phosphorylated and the unphosphorylated B23 molecule. The epitope recognized by the antibody lies within the 68 amino acids at the C-terminus of B23.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication). Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23.

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