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B0681 Sigma

Anti-BACE 1, N-Terminus (46-62) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-β-Site APP Cleaving Enzyme

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Neurobiology, Antibodies for Neurodegenerative Diseases Research,
species reactivity   human
application(s)   western blot: 1:1,000 using a whole cell extract from the human kidney HEK293 cell line stably transfected with human BACE-1
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 60-75 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BACE1(23621)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminus of human BACE-1 (amino acids 46-62).

General description

Alzheimer’s disease is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits composed primarily of the amyloid-βpeptide (Aβ). It has been suggested that Aβ plays a central role in Alzheimer’s disease pathogenesis. Formation of Aβ requires proteolytic cleavage of the β-amyloid precursor protein (APP) by two proteases, β-secretase and γ-secretase. Cleavage by β-secretase at the amino-terminus of Aβ, between residues 670 and 671 of APP, leads to the generation and extracellular release of APPs-β, an approximately 100 kDa soluble N-terminal fragment, and a membrane associated C-terminal fragment bearing the complete Aβ domain. Cleavage of the C-terminal fragment by γ-secretase, which appears to be identical to the presenilins, leads to the formation of Aβ. The membrane-associated aspartic protease BACE-1 (β-site APP cleaving enzyme, Asp2 or memapsin 2) has been identified as β-secretase. A close homologue was also identified and termed BACE-2, Asp1, DRAP or memapsin 1. BACE-1 constitutes the predominant β-secretase activity in human brain tissue. It is highly expressed in neurons, the major site of Aβ generation, while BACE-2 is widely expressed in peripheral tissues.

Anti-BACE-1 (EE-17) recognizes human β-site APP Cleaving Enzyme/β-secretase (broad band at 60-75 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Rabbit polyclonal anti-BACE 1, N-Terminus (46-62) antibody is used to tag β-site APP Cleaving Enzyme/β-secretase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of β-site APP Cleaving Enzyme/β-secretase in the processing of β-amyloid precursor protein (APP) into Aβ and Alzheimer’s disease. Anti-BACE1 is used in techniques such as immunoblotting. Staining of BACE-1 in immunoblotting is specifically inhibited with BACE-1 immunizing peptide (human, amino acids 46-62, with C-terminally added lysine).

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