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  • B0681 - Anti-BACE 1, N-Terminus (46-62) antibody produced in rabbit

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B0681 Sigma

Anti-BACE 1, N-Terminus (46-62) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-β-Site APP Cleaving Enzyme

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Neurobiology, Antibodies for Neurodegenerative Diseases Research,
species reactivity   human
application(s)   western blot: 1:1,000 using a whole cell extract from the human kidney HEK293 cell line stably transfected with human BACE-1
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 60-75 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BACE1(23621)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminus of human BACE-1 (amino acids 46-62).

General description

Alzheimer’s disease is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits composed primarily of the amyloid-βpeptide (Aβ). It has been suggested that Aβ plays a central role in Alzheimer’s disease pathogenesis. Formation of Aβ requires proteolytic cleavage of the β-amyloid precursor protein (APP) by two proteases, β-secretase and γ-secretase. Cleavage by β-secretase at the amino-terminus of Aβ, between residues 670 and 671 of APP, leads to the generation and extracellular release of APPs-β, an approximately 100 kDa soluble N-terminal fragment, and a membrane associated C-terminal fragment bearing the complete Aβ domain. Cleavage of the C-terminal fragment by γ-secretase, which appears to be identical to the presenilins, leads to the formation of Aβ. The membrane-associated aspartic protease BACE-1 (β-site APP cleaving enzyme, Asp2 or memapsin 2) has been identified as β-secretase. A close homologue was also identified and termed BACE-2, Asp1, DRAP or memapsin 1. BACE-1 constitutes the predominant β-secretase activity in human brain tissue. It is highly expressed in neurons, the major site of Aβ generation, while BACE-2 is widely expressed in peripheral tissues.

Anti-BACE-1 (EE-17) recognizes human β-site APP Cleaving Enzyme/β-secretase (broad band at 60-75 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Rabbit polyclonal anti-BACE 1, N-Terminus (46-62) antibody is used to tag β-site APP Cleaving Enzyme/β-secretase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of β-site APP Cleaving Enzyme/β-secretase in the processing of β-amyloid precursor protein (APP) into Aβ and Alzheimer’s disease. Anti-BACE1 is used in techniques such as immunoblotting. Staining of BACE-1 in immunoblotting is specifically inhibited with BACE-1 immunizing peptide (human, amino acids 46-62, with C-terminally added lysine).

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

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