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B1155 Sigma

bisBenzimide H 33258

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Synonym: 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072

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Properties

Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Bioactive Small Molecule Alphabetical Index,
InChI Key   OWRSPPSBNWJJAR-UHFFFAOYSA-N
product line   BioReagent
assay   ≥98% (HPLC and TLC)
form   powder
solubility   H2O: soluble10 mg/mL
  water and ethanol: soluble10 mg/mL
suitability   passes application test for fluorescence
  suitable for cell culture
storage temp.   −20°C

Description

Packaging

100 mg in glass bottle

25 mg in poly bottle

Application

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

Price and Availability


Flow Cytometry Instruments

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SM1140500
Protocols & Articles

Articles

Mycoplasma Detection/Elimination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cel...
Don Finley
BioFiles v7 n1, 2011, 14–15
Keywords: Eliminations, Metabolism, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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