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B1267 Sigma

Biotin-maleimide

≥95% (TLC), powder

Synonym: N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide

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Properties

Related Categories Biotinylation, Molecular Biology, Protein Modification, Protein Structural Analysis, Proteomics,
InChI Key   VAPZWEFPQRDVHR-NJSLBKSFSA-N
assay   ≥95% (TLC)
form   powder
solubility   acetic acid: soluble20 mg/mL
storage temp.   2-8°C

Description

Application

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.

Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

General description

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.

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Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

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