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  • B1531 - Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse

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B1531 Sigma

Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse

clone PT-66, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins,
biological source   mouse
antibody form   purified immunoglobulin
clone   PT-66, monoclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:50,000
  dot blot: 1:32,000
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

phosphotyrosine conjugated to BSA

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Monoclonal anti-phosphotyrosine−biotin antibody can be used in western blotting for analysis of in vitro cultured spleen cell extracts. It can also be used in direct ELISA (diluted1:50,000).

General description

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biotin antibody can be used in dot blot (diluted 1:32,000). Mouse anti-phosphotyrosine?biotin antibody reacts specifically with phosphorylated tyrosine both as the free amino acid or when conjugated to BSA or KLH. This product does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine and threonine, or with phosphorylated molecules like AMP or ATP.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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