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B1656 Sigma

Monoclonal Anti-Rat Kappa & Lambda Light Chains−Biotin antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Rat Kappa & Lambda Light Chains

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Properties

Related Categories Alphabetical Index, Antibodies, Biotin Antibodies, Primary Antibodies, RAE-RAX,
biological source   mouse
antibody form   purified immunoglobulin
clone   RT-39/RL-6, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
species reactivity   rat
does not react with   sheep, cat, chicken, dog, goat, horse, mouse, rabbit, human, pig
application(s)   direct ELISA: 1:40,000
  dot blot: 1:4,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:600
  western blot (chemiluminescent): 1:40,000
isotype   IgG1/IgG2a
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

Monoclonal Anti-Rat Kappa & Lambda Light Chains-Biotin antibody produced in mouse may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a working antibody dilution of 1:600. A dilution of 1:4000 is recommended for dot blot. A working antibody dilution of 1:40000 may be used for ELISA and immunoblotting.

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either kappa or the lambda chain. The B cells express one type of light chain during their life time. The coupling of biotin to monoclonal Anti-Rat Kappa & Lambda Light Chains antibody allows for the binding of various labels such as avidin or streptavidin.

General description

Binds all rat IgG1/IgG2a

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

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