|Related Categories||Alphabetical Index, Antibodies, Biotin Antibodies, Clone Index, Primary Antibodies,|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|mol wt||antigen mol wt 25 kDa|
|application(s)||direct ELISA: 1:40,000|
|dot blot: 1:4,000|
|immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:600|
|western blot (chemiluminescent): 1:40,000|
|shipped in||dry ice|
The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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