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B2025 Sigma

Brilliant Blue G - Colloidal Concentrate



Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

This product has been tested for suitability on SDS-PAGE.


Brilliant Blue G – colloidal stains protein in polyacrylamide and agarose gels (IEF) and provides approximately a 10-fold increase in sensitivity (with fixation) over methanol/acetic acid based Brilliant Blue G and R. Fixing (F7264) is required prior to staining.


The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Price and Availability

Stable Isotope
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
Precautionary statements 
UN 1805 8 / PGIII
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
One bottle of the product makes 1 liter of working solution.  The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
What is the solution stability of Product B2025, Brilliant Blue G?
Once diluted the solution should be stable indefinitely.  It should be stored in the refrigerator and shaken well before each use.  The solution tends to precipitate on standing and heat accelerates precipitation.  Therefore, do not leave the bottle at room temperature overnight.
Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
Use with nitrocellulose is not recommended, due to the methanol.
How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
What is the detection limit of Product B2025, Brilliant Blue G?
The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximu...
Keywords: Electrophoresis, Isoelectric focusing, PAGE

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