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B2436 Sigma

BSEP human

membrane preparation, for Vesicular Transport, recombinant, expressed in baculovirus infected Sf9 cells

Synonym: ABCB11, Bile salt export pump

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Drug Transport Proteins & Reagents, Drug and Xenobiotic Metabolism,
recombinant   expressed in baculovirus infected Sf9 cells
form   membrane preparation
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... ABCB11(8647)

Description

Legal Information

Distributed for SOLVO Biotechnology, Inc.

Physical form

Supplied as isolated Sf9 cell membranes containing human BSEP suspended in 50 mM HEPES-Tris, 100 mM KNO3, and 50 mM sucrose, pH 7.4.

Biochem/physiol Actions

The vesicular transport assay determines the interaction of compounds with the BSEP transporter. The interaction is detected by changes in the initial rate of 3H-taurocholic acid transport by BSEP into membrane vesicles purified from Sf9 cells expressing the transporters. Membrane preparations from infected cells always contain some closed membrane vesicles that have an inside-out orientation (5-10% of total lipid). In the case of these inside-out vesicles, transport of substrates across the membrane takes molecules from the surrounding buffer and transports them into the vesicles.

General description

The bile salt export pump (BSEP/ABCB11) belongs to the family of ATP-binding-cassette (ABC) transporters and has also been called the sister of P-glycoprotein (sister Pgp). Most ABC transporters transport substrates across the cell membrane using ATP as an energy source. BSEP is the major bile salt transporter in the liver canalicular membrane and is inhibited by a number of drugs or drug metabolites. This is potentially a significant mechanism for drug-induced cholestasis. Dysfunction of individual bile salt transporters such as BSEP, due to genetic mutation, suppression of gene expression, disturbed signaling, or steric inhibition, is an important cause of cholestatic liver disease.

The quantity of transported molecules can be determined by methods such as HPLC, LC/MS/MS separation and detection, and also by labeling with fluorescent or radioactive (3H-taurocholic acid) tags. BSEP mediates the transport of taurocholic acid (TC) very efficiently. Compounds that interact with the transporter modulate the initial rate of TC transport measured without any other compounds added. If a substance is a transported substrate of the transporter, it might compete with TC, thus reducing the rate of TC transport. If a compound is an inhibitor of the transporter, it will block the transport of TC into the membrane vesicles. Some compounds can be co-transported with TC, increasing the rate of TC transport compared to the control level.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

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