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B2643 Sigma

Biotinamidohexanoic acid N-hydroxysuccinimide ester

≥98% (TLC), powder

Synonym: (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester, N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester, N-(+)-Biotinyl-6-aminohexanoic acid NHS ester, N-Succinimidyl N-biotinyl-6-aminocaproate, Succinimidyl 6-(biotinamido)hexanoate

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Properties

Related Categories Antibodies, Antibody Labeling, Antibody Modification, Biotin Labeling Reagents, Biotin/Avidin,
InChI Key   UVGHPGOONBRLCX-NJSLBKSFSA-N
assay   ≥98% (TLC)
form   powder
solubility   H2O: ≤2 mg/mL (with sonication)
  DMF: soluble50 mg/mL (Stable up to 2 months in dry DMF.)
storage temp.   −20°C

Description

Packaging

10, 25, 100, 250 mg in poly bottle

Application

Biotinylation reagent incorporating an aminocaproyl spacer. This can reduce the steric hindrance in binding avidin to some biotinylated compounds. Typically coupled to primary amine in the pH range 6.5-8.5.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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