|Related Categories||Antibodies, Antibody Labeling, Antibody Modification, Biotin Labeling Reagents, Biotin/Avidin,|
|solubility||H2O: ≤2 mg/mL (with sonication)|
|DMF: soluble50 mg/mL (Stable up to 2 months in dry DMF.)|
Biotinylation reagent incorporating an aminocaproyl spacer. This can reduce the steric hindrance in binding avidin to some biotinylated compounds. Typically coupled to primary amine in the pH range 6.5-8.5.
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≥98% (HPLC), powder
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≥90% (TLC), powder
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Leary, J.J., et al. Proc. Natl. Acad. Sci. U. S. A. 80, 4045-4049, (1983)
Ligation of Major Histocompatability Complex (MHC) Class I Molecules on Human T Cells Induces Cell Death through PI-3 Kinase–induced c-Jun NH2-terminal Kinase Activity: A Novel Apoptotic Pathway Distinct from Fas-induced Apoptosis Claesson, M.H., et al. J. Cell Biol. 139, 1523-1531, (1997)
Target-sensitive immunoerythrocytes: interaction of biotinylated red blood cells with immobilized avidin induces their lysis by complement. Muzykantov VR, Zaltsman AB, Smirnov MD, et al. Biochim. Biophys. Acta 1279(2), 137-43, (1996)
Monobiotinylated relaxins. Preparation and chemical properties of the mono(biotinyl-epsilon-aminohexanoyl) porcine relaxin. Büllesbach EE and Schwabe C Int. J. Pept. Protein Res. 35(5), 416-23, (1990)
Synthesis and biodistribution of 211At-labeled, biotinylated, and charge-modified poly-L-lysine: evaluation for use as an effector molecule in pretargeted intraperitoneal tumor therapy. Lindegren S, Andersson H, Jacobsson L, et al. Bioconjug. Chem. 13(3), 502-9, (2002)
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