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B2661 Sigma

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym: Anti-fd Bacteriophage

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Properties

Related Categories Alphabetical Index, Antibodies, FB-FJ, Miscellaneous Antibodies, Miscellaneous Antibodies for Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
concentration   ~3.5 mg/mL
application(s)   indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

fd bacteriophage

Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

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Safety Information

WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
The description states Product B2661, Anti-fd Bacteriophage, may be used for staining DNA, chromosomes and nuclei, and for fluorescence microscopy or flow cytometry.  Are there other uses for the product?
The product has also been used for the detection of apoptosis, applied as a label to live cells, as a component of BrdU cell cycle investigation.
What is the expected solution stability for aqueous solutions of Product B2661, Anti-fd Bacteriophage?
Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.
Which is more cell permeable, H33342 or H33258?
H33342 has been found to be about 10 times more cell-permeable than H33258. See Science, 282(5387), 296-298, (1998).
What is the specificity for H33342?
The bisBenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators.  Reference: Loontiens, F. G., et al., Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)] and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry, 29, p. 9029 (1990).
When using Product B2661, Anti-fd Bacteriophage, which is the preferred Hoechst stain for chromosomes?
Hoechst No. 33258 and not Hoechst No. 33342 is the preferable dye for chromosomal staining.
What is the pH of Product B2661, Anti-fd Bacteriophage, in solution?
There is no pH test specification for product B2261 on a lot-to-lot basis.  A one-time measurement of the pH of a 2% solution was 1.9. It has been observed that this material will precipitate from phosphate buffer solutions.
Will chromosomes that are dividing or replicating stain with Product B2661, Anti-fd Bacteriophage?
No; chromosomes that are dividing or replicating will not stain with this dye.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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