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B2661 Sigma

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym: Anti-fd Bacteriophage

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Properties

Related Categories Alphabetical Index, Antibodies, FB-FJ, Miscellaneous Antibodies, Miscellaneous Antibodies for Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
concentration   ~3.5 mg/mL
application(s)   indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

fd bacteriophage

Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
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