|Related Categories||Cloning and Expression, Competent Cells, Core Bioreagents, Life Science Reagents for Cloning, Molecular Biology,|
|shipped in||dry ice|
BL21-T1R competent Escherichia coli is an all-purpose expression strain suitable for the high level production of heterologous proteins directed by various expression vector systems (promoters such as lac, trc, tac, λPL, and araD). BL21-T1R is a B/r strain bacteria, which naturally lacks the lon protease and is also deficient in the outer membrane protease ompT. The absence of proteolytic activity from these two proteases may reduce degradation of some heterologous proteins expressed in the strain. In addition, the tonA genotype confers resistance to the lytic bacteriophages T1 and T5 for protection of clonal stocks.
Sigma′s BL21-T1R competent E. coli cells are grown and made chemically competent using an optimized procedure specific to the strain, followed by strain verification and efficiency testing. The cells are provided in frozen 50 μl aliquots for convenience. Each aliquot can be used for a single transformation.
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|pUC19 Control DNA, 10 ng/μL|
|BL21-T1R Competent Cells|
new publication: Bacterial receptors for phages and colicins as constituents of specific transport system, in Receptors and Recognition, Series B, Vol. 3, J. L. Reissig (Ed.), pp. 101-130. (Chapman & Hall, Ltd., London, 1977). Braun, V., and Hantke, K. Receptors and Recognition, (1977) 3, 101-130
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