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B2787 Sigma

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

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Properties

Related Categories Molecular Biology, Molecular Weight Markers, Protein Electrophoresis, Protein MW Markers, Proteomics,
form   lyophilized powder
usage   1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
mol wt   mol wt 6,500-180,000 Da
packaging   vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

This product contains:
• α2-macroglobulin (equine serum)
• β-galactosidase (E. coli)
• phosphorylase b (rabbit muscle)
• catalase (bovine liver)
• alcohol dehydrogenase (yeast)
• carbonic anhydrase (bovine erythrocyte)
• trypsin inhibitor (soy bean)
• lysozyme (chicken egg)
• aprotinin (bovine lung)
Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Other Notes

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitution

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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