Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.
B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Synonym(s):
protein markers, protein molecular weight markers, protein standards
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1 VIAL
$234.00
5 X 1 VIAL
$924.00
$234.00
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About This Item
UNSPSC Code:
12352200
eCl@ss:
34058011
NACRES:
NA.25
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form
lyophilized powder
Quality Level
usage
1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
mol wt
6,500-180,000 Da
packaging
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
storage temp.
−20°C
Related Categories
1 of 4
This Item | M3546 | SDS7B2 | C6210 |
|---|---|---|---|
| form lyophilized powder | form - | form - | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100, 200 | Quality Level 200, 400 | Quality Level 100, 200 |
| usage vial sufficient for 200 applications (on 10 cm × 10 cm mini gels) | usage vial sufficient for 400 applications on large gels (16 × 18 cm), vial sufficient for 800 mini-gels (10 × 10 cm) | usage - | usage sufficient for 20 applications (on 10 cm × 10 cm mini gels) |
| mol wt 6,500-180,000 Da | mol wt - | mol wt 26,600-180,000 Da | mol wt - |
| packaging vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose) | packaging vial of 200 μL | packaging - | packaging - |
Application
For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.
Preparation Note
Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.
Other Notes
Contains nine biotinylated proteins (approx 0.1 mg total).
This product contains:
- α2-macroglobulin (equine serum)
- β-galactosidase (E. coli)
- phosphorylase b (rabbit muscle)
- catalase (bovine liver)
- alcohol dehydrogenase (yeast)
- carbonic anhydrase (bovine erythrocyte)
- trypsin inhibitor (soy bean)
- lysozyme (chicken egg)
- aprotinin (bovine lung)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)
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The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C. What information can you provide?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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