EMAIL THIS PAGE TO A FRIEND
B2904 Sigma

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-3-indolyl β-D-galactopyranoside, Bluo-Gal

Purchase

Properties

Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,
grade   for molecular biology
InChI Key   LINMATFDVHBYOS-MBJXGIAVSA-N
sterility   non-sterile
assay   ≥98% (HPLC)
form   powder
solubility   DMF: soluble
suitability   suitable for substrate for β-galactosidase
storage temp.   −20°C

Description

Application

Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Packaging

100, 500 mg in poly bottle

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

C4478 S-Gal®/LB Agar Blend, reagent for selection of recombinant bacterial clones
94433 β-Galactosidase stain
B3928 Blue-White Select Screening Reagent, for selection of recombinant bacterial clones
B8931 Magenta-Gal, reagent for selection of recombinant bacterial clones
S9811 S-Gal®, reagent for selection of recombinant bacterial clones
S1813 S-Gal®/LB Agar/Kanamycin Blend, powder
S7313 S-Gal® sodium salt, reagent for selection of recombinant bacterial clones
16658 X-GalNAc, reagent for selection of recombinant bacterial clones
16669 Magenta-Gal, reagent for selection of recombinant bacterial clones
B6650 X-GlcA, reagent for selection of recombinant bacterial clones

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?