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B3170 Sigma

Monoclonal Anti-Bcl-2 antibody produced in mouse

clone Bcl-2-100, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
does not react with   mouse
application(s)   electron microscopy: suitable
  immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  microarray: suitable
  western blot: 1:1,000 using human HeLa cell extract
clone   Bcl-2-100, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 26 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BCL2(596)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to residues 41-54 of the Bcl-2 protein, conjugated to thyroglobulin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Bcl-2 antibody produced in mouse is suitable for western blotting at a concentration of 1:1,000 by using human HeLa cell extract. It is also suitable for immunohistochemistry by using formalin-fixed, paraffin-embedded tissue sections or frozen sections. Further, the antibody may be used for the localization of Bcl-2, by implementing numerous assays like- immunocytochemistry, microarray, electron microscopy and immunoprecipitation.

Biochem/physiol Actions

Protooncogene BCL2 encodes a 26 kD integral outer mitochondrial membrane protein expressed in sub-cellular components such as the mitochondria and nucleus. While mitochondrial BCL2 inhibits apoptosis, BCL2 present in the nucleus may activate cell death. It may also regulate autophagy during starvation. Additionally, BCL2 facilitates the survival of central and peripheral neurons grown in culture in the absence of neurotrophic factors.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Peer-Reviewed Papers
15

References

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