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B3183 Sigma

Anti-BID antibody produced in rabbit

Cleavage site [59/60] specific, affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Bcl-2 Family Proteins,
species reactivity   mouse
application(s)   microarray: suitable
  western blot: suitable using mouse L929 cells treated with TNF-α
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 15 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Bid(12122)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to N-terminal of cleavage site [59/60] of mouse BID.

Physical form

Supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/ml BSA (IgG and protease free) and 0.05% sodium azide.

Application

Polyclonal anti-BID antibody produced in rabbit is suitable for western blotting by using mouse L929 cells treated with TNF-α. It is also suitable for microarray.

Biochem/physiol Actions

The protein encoded by this gene is a member of the BCL-2 family of cell death regulators and is localized on to chromosome 22q11.2. BID is activated by caspase 8 in response to Fas/TNF-R1 receptor activation. Activated BID then translocated to mitochondria where it induces cytochrome c release, which in turn activates downstream caspases. It is a BH3 interacting death domain that heterodimerizes with either agonist BAX or antagonist BCL2. BID initiates apoptosis by binding to regulatory sites on prosurvival BCL2 proteins to directly neutralize their function. Additionally, BID interacts with cathepsins play an important role in the actions of Camptothecin on breast cancer cells.

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